Isolation Identification and Characterization of some Penicillium Isolates for Alpha Amylase Activity

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Isolation Identification and Characterization of some Penicillium Isolates for Alpha Amylase Activity

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Title: Isolation Identification and Characterization of some Penicillium Isolates for Alpha Amylase Activity
Author: Dar, Gowhar Hamid (Scholar); Kamili, Azra N. (Supervisor); Ruqeya, Nazir (Co-supervisor)
Abstract: Aninvestigation was carried out to determine the presence of amylase producing Penicillium species in the soils of Harwan area of Srinagar city. Two study sites were choosen from the area.Soil samples were collected from the study sites for the isolation o of Penicillium isolates. Pour plate method involving serial dilution was used for the isolation of fungi. The media used for isolation were Potato Dextrose Agar(PDA), Rose Bengal agar (RBA) and Malt Extract Agar (MEA). A total of 4 Penicillium isolates were obtained during the study, which showed positive alpha amylase activity, all belonging to division Ascomycota of kingdom fungi.The highest colony forming unit(cfu/g) of 3.5×103 was found for P.chrysogenum at site II in the month of August.Certain agricultural wastes like mustard oil cake,lin seed oil cake and wheat bran were used as crude ingredients in the submerged fermentation process for the production of amylases. The carbon and nitrogen sources were also altered in the culture media for the production of alpha amylases from the isolated Penicillium species. The results of this investigation show that P.chrysogenum has maximum activity of 550.4U/ml in medium with lin seed oil cake at pH 6 and incubation period of 6 days. P.purpurogenum showed maximum activity of 883.93U/ml infermented medium with mustard oil cake as crude substrateat pH6 incubation period of 6 days. P.caesi colum showed maximum activity of 834.8U/ml in medium with glucose as carbon sour ceat pH 9 and incubation period of 18 days. P.funiculosum showed maximum activity of 4254.4U/ml at pH 6 and incubation period of 18 days with mustard oil cake as crude substrate in the medium.The optimum conditions in the medium which were found best to promote the production of alpha amylase from Penicillium species were, when medium was maintained at pH 9 and incubation period of 18 days with glucose as carbon source followed by medium maintained at pH 9, 6 and incubation period of 18 days with yeast extract as nitrogen source and medium maintained at pH 6 and incubation period of 18 days with mustard oil cake in the medium.
URI: http://dspaces.uok.edu.in/jspui//handle/1/1355
Date: 2013


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